Cancer Research UK will allow the public to make donations 24 hours a day with a trial of contactless enabled shop-fronts.
In what the charity claims is a first, owners will be able to donate £2 payments by tapping a contactless enabled debit or credit card against the window of four of its branches.
Once the payment is accepted an interactive screen will show how the money will impact the fight against cancer.
The service - aimed at simplifying the donation process around World Cancer Day on 4 February - is available at shops in Kensington, Guildford, Marylebone and Brighton from 30 January, and will run until 13 February.
"We are excited to be premiering this world-first use of contactless giving technology in four of our shop windows," said Paul Clarke, director of innovation at Cancer Research UK.
"Cancer Research UK does not receive any government funding for our vital research so rely on the generosity of the public.
"The contactless giving through four of our shop windows is a new and exciting way for people to donate to our work and help us bring forward the day when all cancers are cured."
With Visa predicting over 500 million contactless payments to be made between in the next year, the technology has become more pervasive in recent years, with many retailers - from Marks and Spencer to Boots and Lidl - allowing purchases of up to £20.
Last year, Transport for London announced that it would enable passengers on the London Underground to pay for journeys, with the technology previously rolled out on London buses. Barclaycard even trialled contactless gloves over the Christmas period.
Neil Chapman from Clear Channel, which helped develop the Cancer UK service, commented: "This contactless window display is an important step towards offering brands a contactless payment solution away from their point of sale and works brilliantly with the 'tap donation.'"